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Neurobiol Aging. 2017 Jul;55:115-122. doi: 10.1016/j.neurobiolaging.2017.03.021. Epub 2017 Mar 27.

Immune hyperreactivity of Aβ plaque-associated microglia in Alzheimer's disease.

Author information

1
Section Medical Physiology, Department of Neuroscience, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands; Department of Medical Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
2
Section Medical Physiology, Department of Neuroscience, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
3
Department of Neuropathology, University Medical Center Goettingen, Goettingen, Germany.
4
Laboratory of Neurochemistry and Behavior, Institute Born-Bunge, University of Antwerp, Wilrijk, Belgium; Department of Neurology and Alzheimer Research Center, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
5
Neuroinflammation Disease Biology Unit, Lundbeck Research USA, Paramus, NJ, USA.
6
Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, the Netherlands; Astrocyte biology & Neurodegeneration, Netherlands Institute for Neuroscience, Amsterdam, the Netherlands; Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherlands.
7
Astrocyte biology & Neurodegeneration, Netherlands Institute for Neuroscience, Amsterdam, the Netherlands.
8
Division of Molecular Psychiatry, University Medical Center Goettingen, Goettingen, Germany.
9
Laboratory of Neurochemistry and Behavior, Institute Born-Bunge, University of Antwerp, Wilrijk, Belgium; Department of Neurology and Alzheimer Research Center, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands; Biobank, Institute Born-Bunge, Wilrijk, Belgium.
10
Section Medical Physiology, Department of Neuroscience, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands. Electronic address: h.w.g.m.boddeke@umcg.nl.

Abstract

Alzheimer's disease (AD) is strongly associated with microglia-induced neuroinflammation. Particularly, Aβ plaque-associated microglia take on an "activated" morphology. However, the function and phenotype of these Aβ plaque-associated microglia are not well understood. We show hyperreactivity of Aβ plaque-associated microglia upon systemic inflammation in transgenic AD mouse models (i.e., 5XFAD and APP23). Gene expression profiling of Aβ plaque-associated microglia (major histocompatibility complex II+ microglia) isolated from 5XFAD mice revealed a proinflammatory phenotype. The upregulated genes involved in the biological processes (gene ontology terms) included: "immune response to external stimulus" such as Axl, Cd63, Egr2, and Lgals3, "cell motility", such as Ccl3, Ccl4, Cxcr4, and Sdc3, "cell differentiation", and "system development", such as St14, Trpm1, and Spp1. In human AD tissue with similar Braak stages, expression of phagocytic markers and AD-associated genes, including HLA-DRA, APOE, AXL, TREM2, and TYROBP, was higher in laser-captured early-onset AD (EOAD) plaques than in late-onset AD plaques. Interestingly, the nonplaque parenchyma of both EOAD and late-onset AD brains, the expression of above-mentioned markers were similarly low. Here, we provide evidence that Aβ plaque-associated microglia are hyperreactive in their immune response and phagocytosis in the transgenic AD mice as well as in EOAD brain tissue. We suggest that Aβ plaque-associated microglia are the primary source of neuroinflammation related to AD pathology.

KEYWORDS:

Beta-amyloid; Early-onset Alzheimer's disease; Late-onset Alzheimer's disease; Microglia; Neurodegeneration; Neuroinflammation

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