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Gene. 1988 Jul 15;67(1):21-30.

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.

Author information

1
New England BioLabs, Beverly, MA 01915.

Abstract

Vectors were constructed that allow foreign peptides to be expressed in Escherichia coli as fusion proteins. The peptides are fused to the C terminus of maltose-binding protein (MBP), which allows them to be purified by the MBP's affinity to cross-linked amylose (starch). The fusion protein can be directed to the periplasm by including the leader sequence from the phoA gene on the vector.

PMID:
2843437
DOI:
10.1016/0378-1119(88)90004-2
[Indexed for MEDLINE]

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