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Antonie Van Leeuwenhoek. 2017 Jun;110(6):751-758. doi: 10.1007/s10482-017-0845-3. Epub 2017 Apr 22.

Massilia chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from soil.

Author information

1
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
2
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
3
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. joka@snu.ac.kr.

Abstract

A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7eT, was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33-97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA-DNA hybridisation between TA-C7eT and Massilia haematophila KACC 13771T, Massilia suwonesis KACC 12635T, and Massilia yuzhufengensis KACC 16569T revealed a low level of relatedness (<40%). The low levels of DNA-DNA hybridisation values identified strain TA-C7eT as a novel species in the genus Massilia. The major fatty acids of the strain were C16:0 (37.3%) and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) (40.6%). Q-8 was identified as the only isoprenoid quinone. The polar lipids profile of the strain showed the presence of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminophospholipid and three unidentified lipids. The DNA G+C content of the strain was found to be 63.2 mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain TA-C7eT represents a novel species in the genus Massilia, for which the name Massilia chloroacetimidivorans sp. nov. (=KACC 18674T = NBRC 112463T) is proposed.

KEYWORDS:

DNA–DNA hybridisation; Massilia chloroacetimidivorans; Novel species; Polyphasic taxonomy

PMID:
28434178
DOI:
10.1007/s10482-017-0845-3
[Indexed for MEDLINE]

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