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Hum Cell. 2017 Oct;30(4):258-266. doi: 10.1007/s13577-017-0172-z. Epub 2017 Apr 22.

Lactobacillus paracasei strain 06TCa19 suppresses inflammatory chemokine induced by Helicobacter pylori in human gastric epithelial cells.

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Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa, 252-5201, Japan.
Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto, 259-1193, Japan.
Food and Biotechnology School, Mongolian University of Science and Technology, Ulaanbaatar, 15160, Mongolia.
Mongolian Biotechnology Association, Ulaanbaatar, 15160, Mongolia.
Laboratory for Infectious Diseases, School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Research and Development Division, Minami Nihon Rakuno Kyodo Co. Ltd., Takagi 5282, Miyakonojo, Miyazaki, 885-0003, Japan.
Research and Development Division, Minami Nihon Rakuno Kyodo Co. Ltd., Takagi 5282, Miyakonojo, Miyazaki, 885-0003, Japan.


Helicobacter (H.) pylori infection is an important risk factor for gastric cancer that causes gastric inflammation. Inflammatory chemokines such as interleukin (IL)-8 and regulated on activation normal T cell expressed and secreted (RANTES) are elevated in the gastric mucosa by H. pylori. This study aimed to investigate the effects of Lactobacillus paracasei strain 06TCa19, a probiotic strain, on IL-8 and RANTES expression and production induced by H. pylori using human gastric epithelial cell lines. Strain 06TCa19 was shown to suppress H. pylori-mediated elevation of gene expression related to these chemokines in MKN45 cells. The strain also suppressed the increase in IL-8 and RANTES products induced by H. pylori in AGS cells as well as in MKN45 cells. In MKN45 cells inoculated with H. pylori, strain 06TCa19 was shown to downregulate the activation of NF-κB and p38 MAPK signaling pathways. Additionally, the level of the CagA virulence protein of H. pylori in the MKN45 cells and the number of viable H. pylori adhering to MKN45 cells decreased with the addition of strain 06TCa19. Moreover, the strain 06TCa19 notably increased lactic acid in the supernatant of MKN45 cells. Thus, lactic acid released from strain 06TCa19 might have inhibited the adhesion of H. pylori to MKN45 cells and prevented the insertion of H. pylori CagA into the cells, and elevation of IL-8 and RANTES genes and proteins might be suppressed by downregulating the NF-κB and p38 MAPK pathways. Therefore, use of strain 06TCa19 may prevent H. pylori-associated gastric inflammation.


Helicobacter pylori; IL-8; MKN45 cells; Probiotics; RANTES

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