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Toxicol In Vitro. 2017 Aug;42:150-160. doi: 10.1016/j.tiv.2017.04.020. Epub 2017 Apr 19.

Biocorona formation on gold nanoparticles modulates human proximal tubule kidney cell uptake, cytotoxicity and gene expression.

Author information

1
Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State University, Manhattan, KS, United States.
2
Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State University, Manhattan, KS, United States. Electronic address: nmonteiro@ksu.edu.

Abstract

Gold nanoparticles (AuNP) adsorb macromolecules to form a protein corona (PC) after systemic delivery, to which the kidney as the primary excretory organ is constantly exposed. The role of the PC on AuNP cell uptake and toxicity was investigated in vitro in human proximal tubule cells (HPTC) using 40 and 80nm branched polyethylenimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coated AuNP with or without (bare) PCs composed of human plasma (HP) or human serum albumin (HSA) for 0.25 to 24h. Time-dependent intracellular uptake, assessed by ICP-MS showed PC modulated cell uptake and cytotoxicity; with bare 40nm BPEI-AuNP showing the greatest responses. All AuNP showed minimal to no cytokine release. At the nontoxic dose, 40nm bare BPEI-AuNP significantly modified gene expression related to immunotoxicity, steatosis, and mitochondrial metabolism; while at the high dose, pathways of DNA damage and repair, apoptosis, fatty acid metabolism and heat shock response were modulated. HP corona BPEI-AuNP response was comparable to control. These studies clearly showed reduced uptake and cytotoxicity, as well as differentiated gene expression of AuNP with PCs, questioning the utility of in vitro studies using bare NP to assess in vivo effects. Significantly, only cationic bare BPEI-AuNP had HPTC uptake or cytotoxicity suggesting the relative safety of PEG and LA-AuNP as nanomedicine constructs.

KEYWORDS:

Cellular uptake; Gene expression; Gold nanoparticles; Human proximal tubule cells; Mechanisms of toxicity; Protein corona

PMID:
28433809
DOI:
10.1016/j.tiv.2017.04.020
[Indexed for MEDLINE]

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