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Virology. 2017 Jul;507:135-139. doi: 10.1016/j.virol.2017.04.011. Epub 2017 Apr 19.

A humanized mouse-based HIV-1 viral outgrowth assay with higher sensitivity than in vitro qVOA in detecting latently infected cells from individuals on ART with undetectable viral loads.

Author information

1
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA.
2
Division of Experimental Medicine, UCSF School of Medicine, San Francisco, CA 94110, USA; Division of Infectious Diseases, Brigham and Women's Hospital/Harvard Medical School, Boston, MA 02110, USA.
3
Division of Experimental Medicine, UCSF School of Medicine, San Francisco, CA 94110, USA.
4
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA. Electronic address: akkina@colostate.edu.

Abstract

Assays that can verify full viral eradication are essential in the context of achieving a cure for HIV/AIDS. In vitro quantitative viral out growth assays (qVOA) are currently the gold standard for measuring latent HIV-1 but these assays often fail to detect very low levels of replication-competent virus. Here we investigated an alternative in vivo approach for sensitive viral detection using humanized mice (hmVOA). Peripheral blood CD4+ T cell samples from HIV subjects on stable ART with undetectable viral loads by RT-PCR were first assayed by in vitro qVOA. Corresponding patient samples in which no virus was detected by qVOA were injected into humanized mice to allow viral outgrowth. Of the five qVOA virus negative samples, four gave positive viral outgrowth in the hmVOA assay suggesting that it is more sensitive in detecting latent HIV-1.

KEYWORDS:

Comparison of HIV qVOA and in vivo hu-mouse VOA; Humanized mouse-based latent HIV-1 viral outgrowth assay; In vivo latent HIV-1 viral outgrowth assay; Latent HIV-1 detection by hu-mouse-based VOA; Ultra-Sensitive latent HIV-1 detection in patient cells

PMID:
28432928
PMCID:
PMC5901664
DOI:
10.1016/j.virol.2017.04.011
[Indexed for MEDLINE]
Free PMC Article

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