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Arch Microbiol. 2017 Oct;199(8):1103-1112. doi: 10.1007/s00203-017-1378-x. Epub 2017 Apr 21.

Modulation of the multidrug efflux pump EmrD-3 from Vibrio cholerae by Allium sativum extract and the bioactive agent allyl sulfide plus synergistic enhancement of antimicrobial susceptibility by A. sativum extract.

Author information

1
Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA.
2
Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA. manuel.varela@enmu.edu.

Abstract

The causative agent of cholera, Vibrio cholerae, is a public health concern. Multidrug-resistant V. cholerae variants may reduce chemotherapeutic efficacies of severe cholera. We previously reported that the multidrug efflux pump EmrD-3 from V. cholerae confers resistance to multiple structurally distinct antimicrobials. Medicinal plant compounds are potential candidates for EmrD-3 efflux pump modulation. The antibacterial activities of garlic Allium sativum, although poorly understood, predicts that a main bioactive component, allyl sulfide, modulates EmrD-3 efflux. Thus, we tested whether A. sativum extract acts in synergy with antimicrobials and that a main bioactive component allyl sulfide inhibits EmrD-3 efflux. We found that A. sativum extract and allyl sulfide inhibited ethidium bromide efflux in cells harboring EmrD-3 and that A. sativum lowered the MICs of multiple antibacterials. We conclude that A. sativum and allyl sulfide inhibit EmrD-3 and that A. sativum extract synergistically enhances antibacterial agents.

KEYWORDS:

Allium sativum; Allyl sulfide; Antimicrobial resistance; Bacteria; Garlic; Medicinal plants; Multidrug efflux pump; Synergy; Vibrio cholerae

PMID:
28432381
DOI:
10.1007/s00203-017-1378-x
[Indexed for MEDLINE]

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