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Biosens Bioelectron. 2017 Sep 15;95:100-105. doi: 10.1016/j.bios.2017.04.014. Epub 2017 Apr 13.

RNA aptamer-based electrochemical aptasensor for C-reactive protein detection using functionalized silica microspheres as immunoprobes.

Author information

1
College of Chemistry and Materials Science, the Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chem-Biosensing, Anhui Normal University, Wuhu 241000, People's Republic of China.
2
College of Chemistry and Materials Science, the Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chem-Biosensing, Anhui Normal University, Wuhu 241000, People's Republic of China. Electronic address: zhyz65@mail.ahnu.edu.cn.

Abstract

C-reactive protein (CRP) is a widely accepted biomarker of cardiovascular disease and inflammation. In this study, a RNA aptamer-based electrochemical sandwich type aptasensor for CRP detection was described using the functionalized silica microspheres as immunoprobes. Silica microspheres (Si MSs), which have good monodispersity and uniform shape, were firstly synthesized. The silica microspheres functionlized with gold nanoparticles (Au NPs) provided large surface area for immobilizing signal molecules (Zinc ions, Zn2+) and antibodies (Ab). RNA aptamers, which were specific recognized to CRP, were assembled on the surface of Au NPs modified electrode via gold-sulfur affinity. In the presence of CRP, a sandwich structure of aptamer-CRP-immunoprobe was formed. Square wave voltammetry (SWV) was employed to record the sensing signal, and a clearly reductive peak corresponding to Zn2+ at about -1.16V (vs. SCE) was obtained. Under optimal conditions, the aptasensor showed wide linear range (0.005ngmL-1 to 125ngmL-1) and low detection limit (0.0017ngmL-1 at a signal-to-noise ratio of 3). Some possible interfering substance was also investigated, and the results obtained showed that the aptasensor possessed good selectivity. When the aptasensor was applied to real serum samples analysis, the satisfied results were obtained, indicating that the aptasensor possessed potential real application ability.

KEYWORDS:

C-reactive protein; Electrochemical aptasensor; Gold nanoparticles; RNA aptamer; Silica microspheres; Square wave voltammetry

PMID:
28431362
DOI:
10.1016/j.bios.2017.04.014
[Indexed for MEDLINE]

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