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J Agric Food Chem. 2017 May 10;65(18):3636-3646. doi: 10.1021/acs.jafc.6b05161. Epub 2017 May 2.

(2R,3S,2″R,3″R)-Manniflavanone Protects Proliferating Skeletal Muscle Cells against Oxidative Stress and Stimulates Myotube Formation.

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Department of Animal and Veterinary Science, University of Idaho , Moscow, Idaho 83844, United States.
Chair of Food Chemistry and Molecular Sensory Science, Technische Universität München , Lise-Meitner Strasse 34, D-85354 Freising, Germany.
Research Institute, Dongkwang Pharmaceutical Company, Ltd. , Toegye-ro, Jung-gu, Seoul 04535, Republic of Korea.
Department of Biomedical Sciences, Center for Creative Biomedical Scientists at Chonnam National University , Gwangju 61469, Republic of Korea.
School of Biomedical Sciences, Charles Sturt University , Bathurst, New South Wales 2795, Australia.
Korea Food Research Institute , Sungnamsi 13539, Republic of Korea.
Department of Biological Sciences, University of Idaho , Moscow, Idaho 83844, United States.


We investigated the antioxidative properties of (2R,3S,2″R,3″R)-manniflavanone (MF) using in vitro assays and examined its effects on myogenesis and lactate-induced oxidative stress in C2C12 cells. MF was purified from Garcinia buchananii stem bark. H2O2 and oxygen radical absorbance capacity assays demonstrated that MF is a powerful antioxidant. This finding was supported by diphenylpicrylhydrazine radical scavenging activity of MF. MF was less cytotoxic to C2C12 cells compared to ascorbic acid and myricetin. Moreover, MF accelerated myotube formation in the differentiated C2C12 cells by up-regulating myogenic proteins such as MyoG and myosin heavy chain. Furthermore, MF rescued late differentiation of myoblast suppressed by lactate treatment and up-regulated the expression levels of Nrf2 in lactate-induced oxidative stress, indicating that MF stimulates antioxidative activity inside C2C12 cells. Collectively, MF is a potent antioxidant with a higher safety profile than ascorbic acid and myricetin. It reduces oxidative stress-induced delaying of skeletal muscle differentiation by scavenging reactive oxygen species and regulating myogenic proteins factors.


Garcinia; antioxidant; flavonoid; myogenesis; reactive oxygen species

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