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Cancer Gene Ther. 2017 May;24(5):221-226. doi: 10.1038/cgt.2017.10. Epub 2017 Apr 21.

lncRNA-HIT promotes cell proliferation of non-small cell lung cancer by association with E2F1.

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Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, China.
Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, China.
Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, China.


Lung cancer is the leading cause of cancer-related death around the world. Long noncoding RNA (lncRNA) has pivotal roles in cancer occurrence and development. However, only a few lncRNAs have been functionally characterized. In the present study, we investigated the effects of lncRNA-HIT (HOXA transcript induced by TGFβ) expression on non-small cell lung cancer (NSCLC) cell phenotype with the gain-of-function and loss-of-function assays. We found that ectopic expression or knockdown of lncRNA-HIT markedly increased or decreased NSCLC cell proliferation, respectively. Moreover, we also showed that lncRNA-HIT interacted with E2F1 to regulate its target genes, such as Survivin, FOXM1, SKP2, NELL2 and DOK1. Collectively, our findings indicated that lncRNA-HIT affected the proliferation of NSCLC cells at least in part via regulating the occupancy of E2F1 in the promoter regions of its target genes. The lncRNA-HIT-E2F1 complex may be a potential target for NSCLC treatment.

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