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Cancer Gene Ther. 2017 May;24(5):221-226. doi: 10.1038/cgt.2017.10. Epub 2017 Apr 21.

lncRNA-HIT promotes cell proliferation of non-small cell lung cancer by association with E2F1.

Author information

1
Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, China.
2
Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, China.
3
Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, China.

Abstract

Lung cancer is the leading cause of cancer-related death around the world. Long noncoding RNA (lncRNA) has pivotal roles in cancer occurrence and development. However, only a few lncRNAs have been functionally characterized. In the present study, we investigated the effects of lncRNA-HIT (HOXA transcript induced by TGFβ) expression on non-small cell lung cancer (NSCLC) cell phenotype with the gain-of-function and loss-of-function assays. We found that ectopic expression or knockdown of lncRNA-HIT markedly increased or decreased NSCLC cell proliferation, respectively. Moreover, we also showed that lncRNA-HIT interacted with E2F1 to regulate its target genes, such as Survivin, FOXM1, SKP2, NELL2 and DOK1. Collectively, our findings indicated that lncRNA-HIT affected the proliferation of NSCLC cells at least in part via regulating the occupancy of E2F1 in the promoter regions of its target genes. The lncRNA-HIT-E2F1 complex may be a potential target for NSCLC treatment.

PMID:
28429752
DOI:
10.1038/cgt.2017.10
[Indexed for MEDLINE]

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