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ACS Appl Mater Interfaces. 2017 May 17;9(19):16026-16034. doi: 10.1021/acsami.7b03645. Epub 2017 May 4.

Bubble-Mediated Ultrasensitive Multiplex Detection of Metal Ions in Three-Dimensional DNA Nanostructure-Encoded Microchannels.

Author information

1
Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University , Xiamen 361005, P. R. China.
2
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University , 500 Dongchuan Road, Shanghai 200241, P. R. China.
3
School of Laboratory Medicine, Hubei University of Chinese Medicine , Wuhan 430065, P. R. China.
4
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.
5
Department of Pharmaceutical Science, Åbo Akademic University , FI-20520 Turku, Finland.

Abstract

The development of rapid and sensitive point-of-test devices for on-site monitoring of heavy-metal contamination has great scientific and technological importance. However, developing fast, inexpensive, and sensitive microarray sensors to achieve such a goal remains challenging. In this work, we present a DNA-nanostructured microarray (DNM) with a tubular three-dimensional sensing surface and an ordered nanotopography. This microarray enables enhanced molecular interaction toward the rapid and sensitive multiplex detection of heavy-metal ions. In our design, the use of DNA tetrahedral-structured probes engineers the sensing interface with spatially resolved and density-tunable sensing spots that improve the microconfined molecular recognition. A bubble-mediated shuttle reaction was used inside the DNM-functionalized microchannel to improve the target-capturing efficiency. Using this novel DNM biosensor, the sensitive and selective detection of multiple heavy-metal ions (i.e., Hg2+, Ag+, and Pb2+) was achieved within 5 min, the detection limit was down to 10, 10, and 20 nM for Hg2+, Ag+, and Pb2+, respectively. The feasibility of our DNM sensor was further demonstrated by probing heavy-metal ions in real water samples with a direct optical readout. Beyond metal ions, this unique DNM sensor can easily be extended to in vitro bioassays and clinical diagnostics.

KEYWORDS:

DNA nanostructures; metal ions; microarray; microchannel; multiplex detection

PMID:
28429586
DOI:
10.1021/acsami.7b03645
[Indexed for MEDLINE]

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