Format

Send to

Choose Destination
Lipids. 2017 Jun;52(6):499-511. doi: 10.1007/s11745-017-4253-2. Epub 2017 Apr 20.

Short Term High Fat Diet Induces Obesity-Enhancing Changes in Mouse Gut Microbiota That are Partially Reversed by Cessation of the High Fat Diet.

Author information

1
Laboratory of Integrative Biology, CCARM, St. Boniface Hospital Research Centre, 351 Tache Avenue, Winnipeg, MB, R2H 2A6, Canada.
2
Department of Animal Science, Winnipeg, Canada.
3
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
4
Department of Pharmacology and Pharmacy, University of Hong Kong, Hong Kong, China.
5
Agriculture and Agri-Food Canada, Winnipeg, Canada.
6
Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Canada.
7
Laboratory of Integrative Biology, CCARM, St. Boniface Hospital Research Centre, 351 Tache Avenue, Winnipeg, MB, R2H 2A6, Canada. karmino@sbrc.ca.
8
Department of Animal Science, Winnipeg, Canada. karmino@sbrc.ca.
9
Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Canada. karmino@sbrc.ca.

Abstract

The gut microbiota is proposed as a "metabolic organ" involved in energy utilization and is associated with obesity. Dietary intervention is one of the approaches for obesity management. Changes in dietary components have significant impacts on host metabolism and gut microbiota. In the present study, we examined the influence of dietary fat intervention on the modification of gut mucosa-associated microbiota profile along with body weight and metabolic parameter changes. Male C57BL/6J mice (6-week old) were fed a low fat diet (10% kcal fat) as a control or a high fat diet (HFD 60% kcal fat) for 7 weeks. In another group, mice were fed HFD for 5 weeks followed by low fat control diet for 2 weeks (HFD + Control). At 7 weeks, body weight gain, blood glucose and hepatic triacylglycerol levels of mice fed a HFD were significantly higher than that of the control group and the HFD + Control group. There were significant differences in the diversity and predicted functional properties of microbiota in the cecum and colon mucosa between the control group and the HFD group. HFD feeding reduced the ratio of Bacteroidetes to Firmicutes, a microbiota pattern often associated with obesity. The HFD + Control diet partially restored the diversity and composition of microbiota in the cecum to the pattern observed in mice fed a control diet. These results suggest that short-term high fat diet withdrawal can restore metabolic changes and prevent excess body weight gain, however, long-term dietary intervention may be required to optimize the restoration of gut microbiota in mouse.

KEYWORDS:

Dietary fat intervention; Gut microbiota; High fat diet; Mucosa

PMID:
28429150
DOI:
10.1007/s11745-017-4253-2
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center