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Pathog Immun. 2017;2(1):23-33. doi: 10.20411/pai.v2i1.99. Epub 2017 Feb 1.

A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST.

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Medical and Research Services Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH.
North Dakota State University, Fargo, ND.
Public Health Research Institute Tuberculosis Center, Rutgers University, Newark, NJ.
J. Craig Venter Institute, La Jolla, CA.
Department of Medicine, Infection Control and Prevention Unit of Infectious Diseases, Assaf Harofeh Medical Center.
Sanford Health, Fargo, Department of Infectious Disease, Fargo, ND.
Wayne State University and Detroit Medical Center, Detroit, MI.
Rush University Medical Center, Chicago, IL.
Centro Internacional de Entrenamiento e Investigaciones M├ędicas (CIDEIM), Cali, Colombia.
Departments of Medicine, Microbiology and Cellular Biology, and Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH.


Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.


Enterobacter cloacae; MLST; bacterial typing; hsp60; infectious diseases outbreaks; molecular epidemiology; rep-PCR

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