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Pathog Immun. 2017;2(1):23-33. doi: 10.20411/pai.v2i1.99. Epub 2017 Feb 1.

A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST.

Author information

1
Medical and Research Services Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH.
2
North Dakota State University, Fargo, ND.
3
Public Health Research Institute Tuberculosis Center, Rutgers University, Newark, NJ.
4
J. Craig Venter Institute, La Jolla, CA.
5
Department of Medicine, Infection Control and Prevention Unit of Infectious Diseases, Assaf Harofeh Medical Center.
6
Sanford Health, Fargo, Department of Infectious Disease, Fargo, ND.
7
Wayne State University and Detroit Medical Center, Detroit, MI.
8
Rush University Medical Center, Chicago, IL.
9
Centro Internacional de Entrenamiento e Investigaciones M├ędicas (CIDEIM), Cali, Colombia.
10
Departments of Medicine, Microbiology and Cellular Biology, and Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH.

Abstract

Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.

KEYWORDS:

Enterobacter cloacae; MLST; bacterial typing; hsp60; infectious diseases outbreaks; molecular epidemiology; rep-PCR

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