Format

Send to

Choose Destination
J Oral Pathol Med. 2017 Oct;46(9):759-765. doi: 10.1111/jop.12585. Epub 2017 May 14.

Role of sialyl 6-sulfo Lewis X in antitumor immunity against oral squamous cell carcinoma.

Author information

1
Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan.
2
Department of Dentistry and Oral Surgery, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan.
3
Division of Surgical Pathology, University of Fukui Hospital, Eiheiji, Japan.

Abstract

BACKGROUND:

Tumor-infiltrating lymphocytes (TILs) reportedly play a pivotal role in antitumor immunity against oral squamous cell carcinoma (OSCC); however, mechanisms governing TIL recruitment to OSCC tissues remain to be clarified. This study was undertaken to assess a potential association between TILs and high endothelial venule (HEV)-like vessels that express sialyl 6-sulfo Lewis X (LeX).

METHODS:

OSCC tissue sections (n=41) were subjected to immunohistochemistry for sialyl 6-sulfo LeX and CD34 to allow quantitation of HEV-like vessels. Triple immunohistochemistry for sialyl 6-sulfo LeX and either CD3 and CD20 or CD4 and CD8 was conducted to determine which lymphocyte subset is more closely associated with HEV-like vessels.

RESULTS:

HEV-like vessels expressing sialyl 6-sulfo LeX were detected in 27 of 41 (65.9%) OSCC cases, and these vessels were more frequently found in early disease (T1/T2 stages) compared with advanced (T3/T4) stages. The number of T cells attached to the inner surface of these HEV-like vessels was significantly greater than that of B cells, while the number of CD4+ helper T cells and CD8+ cytotoxic T cells did not differ significantly. Interestingly, sialyl 6-sulfo LeX was also expressed on the membrane of a fraction of OSCC cells, and CD8+ cytotoxic T cells were almost exclusively found attached to these carcinoma cells.

CONCLUSIONS:

Sialyl 6-sulfo LeX is displayed not only on HEV-like vessels but also on OSCC cells and may potentially function in antitumor immunity against OSCC.

KEYWORDS:

antitumor immunity; high endothelial venule; oral squamous cell carcinoma; sialyl 6-sulfo Lewis X; tumor-infiltrating lymphocyte

PMID:
28425129
DOI:
10.1111/jop.12585
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center