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Oncotarget. 2017 Apr 18;8(16):27328-27338. doi: 10.18632/oncotarget.16033.

Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) associated with poor prognosis of head and neck carcinomas.

Author information

1
Department of Oral Pathology, Institute of Dentistry, University of Turku, Turku, Finland.
2
Department of Pathology, Turku University Central Hospital, Turku, Finland.
3
Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, Turku, Finland.
4
Department of Clinical Research, Biohit HealthCare Oyj, Helsinki, Finland.

Abstract

BACKGROUND:

Epstein-Barr virus (EBV) is the main cause of nasopharyngeal carcinoma (NPC), also found in other head and neck carcinomas (HNSCCs) where its role remains controversial.

RESULTS:

EBV was found in 80% and 21% of the samples with PCR and ISH (in cancer cells), respectively. Eight of ISH-positive samples were not NPCs. EBER-RNA detection in carcinoma cells was associated with worse prognosis, whether or not NPCs were included. HPV/EBV and HSV/HPV coinfections associated with a shorter survival. LMP-1 expression, positive in 51% of samples did not correlate with the disease outcome.

MATERIALS AND METHODS:

We analyzed EBV in 73 HNSCC samples with a known HPV and HSV-1 status, using in situ hybridization (ISH) and immunohistochemistry (IHC) for EBV-early transcripts (EBER) and LMP-1 protein, respectively. EBV-DNA was detected with a Luminex-based method. The results were correlated with HPV-status and disease outcome.

CONCLUSIONS:

EBV is transcriptionally active in NPC cells but also in a subgroup of other HNSCCs.

KEYWORDS:

EBV; Epstein-Barr virus; HPV; HSV-1; head and neck cancer

PMID:
28423694
PMCID:
PMC5432338
DOI:
10.18632/oncotarget.16033
[Indexed for MEDLINE]
Free PMC Article

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