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FEBS Lett. 2017 May;591(10):1360-1370. doi: 10.1002/1873-3468.12659. Epub 2017 May 7.

Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis.

Author information

1
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan.
2
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Chiyoda-ku, Tokyo, Japan.
3
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.
4
Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa, Kanagawa, Japan.

Abstract

The phytoflagellate Euglena gracilis produces a large amount of paramylon (PM), a conglomerate of liner β-1,3-glucan chains, as a storage polysaccharide. PM is synthesized from uridine diphosphate-glucose, but its mechanism of formation is largely unknown. Two enzymes, glucan synthase-like (EgGSL) 1 and EgGSL2 were previously identified as candidates for PM synthesis in a Euglena transcriptome analysis. Here, we performed a reverse genetic analysis on these enzymes. Knockdown of EgGSL2, but not EgGSL1, significantly inhibits PM accumulation in Euglena cells. Additionally, β-1,3-glucan synthesis is detected in a PM-associated membrane fraction extracted from Euglena cells. Our findings indicate that EgGSL2 is the predominant enzyme for PM biosynthesis.

KEYWORDS:

Euglena gracilis ; paramylon; β-1,3-glucan synthase

PMID:
28423179
DOI:
10.1002/1873-3468.12659
[Indexed for MEDLINE]
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