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Elife. 2017 Apr 19;6. pii: e24336. doi: 10.7554/eLife.24336.

Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development.

Author information

1
Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany.

Abstract

Eukaryotic 14-3-3 proteins have been implicated in the regulation of diverse biological processes by phosphorylation-dependent protein-protein interactions. The Arabidopsis genome encodes two groups of 14-3-3s, one of which - epsilon - is thought to fulfill conserved cellular functions. Here, we assessed the in vivo role of the ancestral 14-3-3 epsilon group members. Their simultaneous and conditional repression by RNA interference and artificial microRNA in seedlings led to altered distribution patterns of the phytohormone auxin and associated auxin transport-related phenotypes, such as agravitropic growth. Moreover, 14-3-3 epsilon members were required for pronounced polar distribution of PIN-FORMED auxin efflux carriers within the plasma membrane. Defects in defined post-Golgi trafficking processes proved causal for this phenotype and might be due to lack of direct 14-3-3 interactions with factors crucial for membrane trafficking. Taken together, our data demonstrate a fundamental role for the ancient 14-3-3 epsilon group members in regulating PIN polarity and plant development.

KEYWORDS:

14-3-3; PIN polarity; auxin transport; plant biology

PMID:
28422008
PMCID:
PMC5397284
DOI:
10.7554/eLife.24336
[Indexed for MEDLINE]
Free PMC Article

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