A multistage-dialysis microdevice for extraction of cryoprotectants

Lili Zou; Shibo Li; Yufeng Kang; Juanjuan Liu; Liqun He; Sijie Sun; Dayong Gao; Bensheng Qiu; Weiping Ding

doi:10.1007/s10544-017-0174-3

A multistage-dialysis microdevice for extraction of cryoprotectants

Biomed Microdevices. 2017 Jun;19(2):30. doi: 10.1007/s10544-017-0174-3.

Authors

Lili Zou^{1

2}, Shibo Li^{1

2}, Yufeng Kang^{1

2}, Juanjuan Liu^{1

2}, Liqun He³, Sijie Sun⁴, Dayong Gao⁵, Bensheng Qiu^{1

2}, Weiping Ding^{6

7}

Affiliations

¹ Center for Biomedical Engineering, University of Science and Technology of China, Hefei, Anhui, 230027, China.
² Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui, 230027, China.
³ Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei, Anhui, 230027, China.
⁴ Department of Laboratory Medicine, University of Washington, Seattle, WA, 98195, USA.
⁵ Department of Mechanical Engineering, University of Washington, Seattle, WA, 98195, USA.
⁶ Center for Biomedical Engineering, University of Science and Technology of China, Hefei, Anhui, 230027, China. wpdings@ustc.edu.cn.
⁷ Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui, 230027, China. wpdings@ustc.edu.cn.

PMID: 28421400
DOI: 10.1007/s10544-017-0174-3

Abstract

In this study, we present a multistage-dialysis microdevice (MDM) for extraction of cryoprotectants (CPAs) from a CPA-laden cell suspension. We confirmed the functions of the key designs of the MDM using a fluorescence solution, we assessed the performance of the MDM by using the MDM to unload glycerin from glycerin-loaded swine erythrocytes, and we investigated the effects of the cell suspension flow rate, glycerin concentration, cell density, and membrane pore size on the clearance efficiency of glycerin (C_G), the survival rate of cells (S_C), and the recovery rate of cells (R_C). Under the designed conditions, C_G, S_C, and R_C reached ~60%, ~90%, and ~70%, respectively. In addition, a high flow rate causes high S_C and R_C but a low C_G. For a low glycerin concentration, C_G, S_C, and R_C are all high. If a low cell density or a large pore membrane is used, C_G is high, whereas both S_C and R_C are low. This work provides insight into the development of microfluidic devices for the inline extraction of cryoprotectants from a small volume of cryopreserved cells prior to the use of the cells in lab-on-a-chip applications.

Keywords: Cryoprotectant; Deglycerolization; Dialysis; Erythrocyte; Microfluidic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cryoprotective Agents / isolation & purification*
Dialysis / instrumentation*
Lab-On-A-Chip Devices*

Substances

Cryoprotective Agents