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Front Microbiol. 2017 Apr 4;8:526. doi: 10.3389/fmicb.2017.00526. eCollection 2017.

Mosquito Microbiome Dynamics, a Background for Prevalence and Seasonality of West Nile Virus.

Author information

1
Faculty of Science, University of South BohemiaCeske Budejovice, Czechia.
2
Biology Centre of ASCR, Institute of ParasitologyCeske Budejovice, Czechia.
3
Department of Biology, University of Massachusetts BostonBoston, MA, USA.
4
Rowland Institute, Harvard UniversityCambridge, MA, USA.
5
Cooperative Institute for Research in Environmental Sciences, University of ColoradoBoulder, CO, USA.
6
Department of Ecology and Evolutionary Biology, University of ColoradoBoulder, CO, USA.
7
Sporometrics IncToronto, ON, Canada.
8
Department of Computer Science and Engineering, Center for Microbiome Innovation, University of California San DiegoLa Jolla, CA, USA.
9
Department of Pediatrics, University of California San DiegoLa Jolla, CA, USA.
10
Division of Occupational and Environmental Health, Dalla Lana School of Public Health, University of TorontoToronto, ON, Canada.

Abstract

Symbiotic microbial communities augment host phenotype, including defense against pathogen carriage and infection. We sampled the microbial communities in 11 adult mosquito host species from six regions in southern Ontario, Canada over 3 years. Of the factors examined, we found that mosquito species was the largest driver of the microbiota, with remarkable phylosymbiosis between host and microbiota. Seasonal shifts of the microbiome were consistently repeated over the 3-year period, while region had little impact. Both host species and seasonal shifts in microbiota were associated with patterns of West Nile virus (WNV) in these mosquitoes. The highest prevalence of WNV, with a seasonal spike each year in August, was in the Culex pipiens/restuans complex, and high WNV prevalence followed a decrease in relative abundance of Wolbachia in this species. Indeed, mean temperature, but not precipitation, was significantly correlated with Wolbachia abundance. This suggests that at higher temperatures Wolbachia abundance is reduced leading to greater susceptibility to WNV in the subsequent generation of C. pipiens/restuans hosts. Different mosquito genera harbored significantly different bacterial communities, and presence or abundance of Wolbachia was primarily associated with these differences. We identified several operational taxonomic units (OTUs) of Wolbachia that drive overall microbial community differentiation among mosquito taxa, locations and timepoints. Distinct Wolbachia OTUs were consistently found to dominate microbiomes of Cx. pipiens/restuans, and of Coquilletidia perturbans. Seasonal fluctuations of several other microbial taxa included Bacillus cereus, Enterococcus, Methylobacterium, Asaia, Pantoea, Acinetobacter johnsonii, Pseudomonas, and Mycoplasma. This suggests that microbiota may explain some of the variation in vector competence previously attributed to local environmental processes, especially because Wolbachia is known to affect carriage of viral pathogens.

KEYWORDS:

Aedes vexans; Culex pipiens; Wolbachia; arbovirus; disease ecology; flaviviridae

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