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Environ Microbiol. 2017 Aug;19(8):3039-3058. doi: 10.1111/1462-2920.13763. Epub 2017 May 11.

The low diverse gastric microbiome of the jellyfish Cotylorhiza tuberculata is dominated by four novel taxa.

Author information

1
Mediterranean Institute for Advanced Studies (IMEDEA; CSIC-UIB), Marine Microbiology Group, Esporles, E-07190, Spain.
2
School of Civil and Environmental Engineering, Georgia Institute of Technology, 311 Ferst Dr. NW, Atlanta, GA, 30332, USA.
3
Ribocon GmbH, Bremen, 28359, Germany.
4
Department of Physiology, Genetics and Microbiology, and Multidisciplinary Institute for Environmental Studies Ramon Margalef, University of Alicante, Alicante, Spain.
5
Department of Life Science, University of Trieste, Via L. Giorgieri 10, Trieste, 34127, Italy.
6
Department of Molecular Ecology, Max-Planck-Institut für Marine Mikrobiologie, Bremen, D-28359, Germany.
7
School of Biological Sciences, Georgia Institute of Technology, 950 Atlantic Dr. NW, Atlanta, GA, 30332, USA.

Abstract

Cotylorhiza tuberculata is an important scyphozoan jellyfish producing population blooms in the Mediterranean probably due to pelagic ecosystem's decay. Its gastric cavity can serve as a simple model of microbial-animal digestive associations, yet poorly characterized. Using state-of-the-art metagenomic population binning and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), we show that only four novel clonal phylotypes were consistently associated with multiple jellyfish adults. Two affiliated close to Spiroplasma and Mycoplasma genera, one to chlamydial 'Candidatus Syngnamydia', and one to bacteroidetal Tenacibaculum, and were at least one order of magnitude more abundant than any other bacteria detected. Metabolic modelling predicted an aerobic heterotrophic lifestyle for the chlamydia, which were found intracellularly in Onychodromopsis-like ciliates. The Spiroplasma-like organism was predicted to be an anaerobic fermenter associated to some jellyfish cells, whereas the Tenacibaculum-like as free-living aerobic heterotroph, densely colonizing the mesogleal axis inside the gastric filaments. The association between the jellyfish and its reduced microbiome was close and temporally stable, and possibly related to food digestion and protection from pathogens. Based on the genomic and microscopic data, we propose three candidate taxa: 'Candidatus Syngnamydia medusae', 'Candidatus Medusoplasma mediterranei' and 'Candidatus Tenacibaculum medusae'.

PMID:
28419691
DOI:
10.1111/1462-2920.13763
[Indexed for MEDLINE]

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