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PLoS One. 2017 Apr 18;12(4):e0176076. doi: 10.1371/journal.pone.0176076. eCollection 2017.

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots.

Author information

1
Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil.
2
Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.
3
Laboratório de Biologia Celular e Tecidual, CBB, UENF, Campos dos Goytacazes, RJ, Brazil.
4
Laboratório de Química e Função de Proteínas e Peptídeos, CBB, UENF, Campos dos Goytacazes, RJ, Brazil.
5
Laboratório de Cultura de Tecidos Vegetais (Biofábrica), Universidade Federal Rural do Rio de Janeiro Campus Campos dos Goytacazes, Campos dos Goytacazes, RJ, Brazil.

Abstract

Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075. The RB855536 cultivar is more tolerant to salt stress than CB38-22. A quantitative label-free shotgun proteomic analysis identified 1172 non-redundant proteins, and 1160 of these were observed in both cultivars in the presence or absence of NaCl. Compared with CB38-22, the RB855536 cultivar showed a greater abundance of proteins involved in non-enzymatic antioxidant mechanisms, ion transport, and photosynthesis. Some proteins, such as calcium-dependent protein kinase, photosystem I, phospholipase D, and glyceraldehyde-3-phosphate dehydrogenase, were more abundant in the RB855536 cultivar under salt stress. Our results provide new insights into the response of sugarcane to salt stress, and the changes in the abundance of these proteins might be important for the acquisition of ionic and osmotic homeostasis during exposure to salt stress.

PMID:
28419154
PMCID:
PMC5395195
DOI:
10.1371/journal.pone.0176076
[Indexed for MEDLINE]
Free PMC Article

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