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PLoS Genet. 2017 Apr 18;13(4):e1006753. doi: 10.1371/journal.pgen.1006753. eCollection 2017 Apr.

The SnRK2 kinases modulate miRNA accumulation in Arabidopsis.

Yan J1,2, Wang P1,2, Wang B2, Hsu CC3, Tang K2, Zhang H2,4, Hou YJ2, Zhao Y1,2, Wang Q2,5, Zhao C1,2, Zhu X1,2, Tao WA3, Li J1, Zhu JK1,2.

Author information

1
Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
2
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, United States of America.
3
Departments of Biochemistry, Purdue University, West Lafayette, Indiana, United States of America.
4
State Key Laboratory of Wheat and Maize Crop Sciences, College of Life Science, Henan Agricultural University, Zhengzhou, China.
5
College of Biosciences and Biotechnology, Hunan Agricultural University, Changsha, China.

Abstract

MicroRNAs (miRNAs) regulate gene expression and play critical roles in growth and development as well as stress responses in eukaryotes. miRNA biogenesis in plants requires a processing complex that consists of the core components DICER-LIKE 1 (DCL1), SERRATE (SE) and HYPONASTIC LEAVES (HYL1). Here we show that inactivation of functionally redundant members of the SnRK2 kinases, which are the core components of abscisic acid (ABA) and osmotic stress signaling pathways, leads to reduction in miRNA accumulation under stress conditions. Further analysis revealed that the steady state level of HYL1 protein in plants under osmotic stress is dependent on the SnRK2 kinases. Additionally, our results suggest that the SnRK2 kinases physically associate with the miRNA processing components SE and HYL1 and can phosphorylate these proteins in vitro. These findings reveal an important role for the SnRK2 kinases in the regulation of miRNA accumulation and establish a mechanism by which ABA and osmotic stress signaling is linked to miRNA biogenesis.

PMID:
28419088
PMCID:
PMC5413060
DOI:
10.1371/journal.pgen.1006753
[Indexed for MEDLINE]
Free PMC Article

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