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J Mass Spectrom. 2017 May;52(5):319-341. doi: 10.1002/jms.3932.

Current trends in quantitative proteomics - an update.

Author information

1
University of Victoria - Genome British Columbia Proteomics Centre, University of Victoria, Victoria, BC, V8Z 7X8, Canada.
2
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, V8P 5C2, Canada.
3
Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, QC, H3T 1E2, Canada.
4
Gerald Bronfman Department of Oncology, Jewish General Hospital, Montreal, Quebec, H3T 1E2, Canada.

Abstract

Proteins can provide insights into biological processes at the functional level, so they are very promising biomarker candidates. The quantification of proteins in biological samples has been routinely used for the diagnosis of diseases and monitoring the treatment. Although large-scale protein quantification in complex samples is still a challenging task, a great amount of effort has been made to advance the technologies that enable quantitative proteomics. Seven years ago, in 2009, we wrote an article about the current trends in quantitative proteomics. In writing this current paper, we realized that, today, we have an even wider selection of potential tools for quantitative proteomics. These tools include new derivatization reagents, novel sampling formats, new types of analyzers and scanning techniques, and recently developed software to assist in assay development and data analysis. In this review article, we will discuss these innovative methods, and their current and potential applications in proteomics.

KEYWORDS:

Orbitrap; SWATH; mass spectrometry; proteomics; quantitation; standardization

PMID:
28418607
DOI:
10.1002/jms.3932
[Indexed for MEDLINE]

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