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Mar Drugs. 2017 Apr 12;15(4). pii: E119. doi: 10.3390/md15040119.

Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 Preadipocytes.

Author information

1
Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea. mkang1@bidmc.harvard.edu.
2
Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02445, USA. mkang1@bidmc.harvard.edu.
3
Department of Animal Bio and Applied Chemistry, Konkuk University, Chungju 27478, Korea. dingyuling@naver.com.
4
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science &Technology (KIOST), Jeju 63349, Korea. euna0718@kioat.ac.kr.
5
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science &Technology (KIOST), Jeju 63349, Korea. choiyk@kiost.ac.kr.
6
Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas-UNICAMP, Campinas 02134, Brazil. thiagomatosaraujo@gmail.com.
7
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science &Technology (KIOST), Jeju 63349, Korea. sjheo@kiost.ac.kr.
8
Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju 27478, Korea. seunghong0815@gmail.com.

Abstract

Seaweed, a popular and abundant food ingredient mainly consumed in Asian countries, is a good source of bioactive compounds with anti-obesity effects. However, the anti-obesity effects of Sargassum thunbergii have not yet been established. In this study, we isolated six indole derivatives (STCs)-indole-2-carboxaldehyde (STC-1), indole-3-carboxaldehyde (STC-2), indole-4-carboxaldehyde (STC-3), indole-5-carboxaldehyde (STC-4), indole-6-carboxaldehyde (STC-5), and indole-7-carboxaldehyde (STC-6)-from S. thunbergii and evaluated their inhibitory effects on adipocyte differentiation in 3T3-L1 cells. We found that STC-1 and STC-5 resulted in non-toxic inhibition of the differentiation of 3T3-L1 adipocytes and thus selected these compounds for further study. STC-1 and STC-5 significantly inhibited lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein 1c (SREBP-1c) in a dose-dependent manner. The specific mechanism mediating the effects of STC-1 and STC-5 was shown to be AMP-activated protein kinase (AMPK) activation. Our results demonstrated the inhibitory effect of STC-1 and STC-5 on adipogenesis through the activation of the AMPK signal pathway. Together, these findings suggested that STC-1 and STC-5 may be effective candidates for the prevention of obesity or obesity-related diseases.

KEYWORDS:

Sargassum thunbergii; adipocyte; anti-obesity effect; indole derivatives

PMID:
28417922
DOI:
10.3390/md15040119
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