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Biosens Bioelectron. 2017 Sep 15;95:21-26. doi: 10.1016/j.bios.2017.04.010. Epub 2017 Apr 12.

Label-free upconversion nanoparticles-based fluorescent probes for sequential sensing of Cu2+, pyrophosphate and alkaline phosphatase activity.

Author information

1
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, People's Republic of China.

Abstract

An efficient near-infrared fluorescence probe has been developed for the sequential detection of Cu2+, pyrophosphate (P2O74-, PPi), and alkaline phosphatase (ALP), which is based on the "off-on-off" fluorescence switch of branched polyethyleneimine (PEI)-capped NaGdF4:Yb/Tm upconversion nanoparticles (UCNPs). The fluorescence is quenched via energy transfer from UCNPs to Cu2+ for the coordination of PEI with Cu2+. The strong affinity between Cu2+ and PPi leads to the formation of Cu2+-PPi complex and results in the detachment of Cu2+ from the surface of UCNPs, thus the fluorescence is triggered on. ALP-directed hydrolysis of PPi causes the disassembly of Cu2+-PPi complex and re-conjugation between Cu2+ with PEI, which leads to the switch-off fluorescence of UCNPs. The system allows sequential analysis of Cu2+, PPi, and ALP by modulating the switch of the fluorescence of UCNPs with detection limits of 57.8nM, 184nM, and 0.019U/mL for Cu2+, PPi, and ALP, respectively. By virtue of the NIR feature and excellent biocompatibility, the UCNPs-based probes are suitable for bioimaging. Taking Cu2+ visualization as a model, the nanoprobes have been successfully applied for intracellular imaging of Cu2+ in living cells.

KEYWORDS:

Alkaline phosphatase; Bioimaging; Copper ions; Fluorescent probe; Pyrophosphate ions; Upconversion nanoparticles

PMID:
28411533
DOI:
10.1016/j.bios.2017.04.010
[Indexed for MEDLINE]

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