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Methods Mol Biol. 2017;1595:291-304. doi: 10.1007/978-1-4939-6937-1_27.

Analysis of Peroxisomal β-Oxidation During Storage Oil Mobilization in Arabidopsis thaliana Seedlings.

Author information

1
Institute for Plant Biochemistry and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University, Universitätsstrasse 1, Düsseldorf, 40225, Germany.
2
Institute for Plant Biochemistry and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University, Universitätsstrasse 1, Düsseldorf, 40225, Germany. Nicole.Linka@hhu.de.

Abstract

Peroxisomal β-oxidation in plants is essential for mobilization of storage oil in seed-oil storing plants, such as Arabidopsis thaliana. In plants, degradation of fatty acids occurs exclusively in peroxisomes via β-oxidation, driving seedling growth and development upon germination. Thus, the determination of storage oil breakdown rates is a useful approach to investigate defects in peroxisomal β-oxidation. Here we describe an acid catalyzed derivatization process of fatty acids representing a fast and efficient procedure to generate high yields of fatty acid methyl esters (FAMEs). The subsequent analysis by gas chromatography coupled to mass spectrometry (GC-MS) allows the quantification of total fatty acid content. The results provide detailed information of the complete storage oil breakdown process via peroxisomal β-oxidation during seedling growth.

KEYWORDS:

Fatty acid β-oxidation; Peroxisomes; Plant seedling; Storage oil mobilization

PMID:
28409472
DOI:
10.1007/978-1-4939-6937-1_27
[Indexed for MEDLINE]

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