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J Phys Chem Lett. 2017 May 4;8(9):1932-1936. doi: 10.1021/acs.jpclett.7b00575. Epub 2017 Apr 17.

Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential.

Author information

1
Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907-2032, United States.
2
Interdisciplinary Life Science Program, Purdue University , West Lafayette, Indiana 47907, United States.
3
Stark Neurosciences Research Institute, Indiana University School of Medicine , Indianapolis, Indiana 46202-2266, United States.
4
Medicinal Chemistry and Molecular Pharmacology, Purdue University , West Lafayette, Indiana 47907, United States.
5
MD PhD Program, Indiana University School of Medicine , Indianapolis, Indiana 46202-5120, United States.
6
Department of Chemistry, Purdue University , West Lafayette, Indiana 47907-2084, United States.

Abstract

Detecting membrane potentials is critical for understanding how neuronal networks process information. We report a vibrational spectroscopic signature of neuronal membrane potentials identified through hyperspectral stimulated Raman scattering (SRS) imaging of patched primary neurons. High-speed SRS imaging allowed direct visualization of puff-induced depolarization of multiple neurons in mouse brain slices, confirmed by simultaneous calcium imaging. The observed signature, partially dependent on sodium ion influx, is interpreted as ion interactions on the CH3 Fermi resonance peak in proteins. By implementing a dual-SRS balanced detection scheme, we detected single action potentials in electrically stimulated neurons. These results collectively demonstrate the potential of sensing neuronal activities at multiple sites with a label-free vibrational microscope.

PMID:
28407470
DOI:
10.1021/acs.jpclett.7b00575
[Indexed for MEDLINE]

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