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Genet Mol Res. 2017 Apr 13;16(2). doi: 10.4238/gmr16029608.

Population genetic characterization of the Japanese oak silkmoth, Antheraea yamamai (Lepidoptera: Saturniidae), using novel microsatellite markers and mitochondrial DNA gene sequences.

Author information

1
Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Republic of Korea.
2
College of Agriculture & Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, Republic of Korea.
3
C & K Genomics Inc., Seoul National University, Seoul, Republic of Korea.
4
College of Agriculture & Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, Republic of Korea ikkim81@chonnam.ac.kr.

Abstract

The Japanese oak silkmoth, Antheraea yamamai Guérin-Méneville, 1861 (Lepidoptera: Saturniidae), is an important natural resource of industrial value for silk fiber production. Owing to a lack of geographic and population genetic information, systematic domestication of An. yamamai has not been possible yet. In this study, 10 microsatellite markers developed using next-generation sequencing and two mitochondrial DNA (mtDNA) gene sequences (COI and ND4) were used to investigate the genetic variation and geographic structure of An. yamamai populations in South Korea. The two mtDNA gene sequences revealed very low total genetic variation and, consequently, low geographic variation, validating the use of more variable molecular markers. Genotyping of 76 An. yamamai individuals from nine localities in South Korea showed that the observed number of alleles at each locus ranged from 3 to 26, the polymorphism information content was 0.2990-0.9014, the observed and expected heterozygosities were 0.3252-0.9076 and 0.2500-0.9054, respectively, and FIS was -0.654-0.520. The population-based FIS, FST, RST, and global Mantel tests all suggested that the An. yamamai populations were overall well-interconnected, suggesting that any population can be used as a genetic source for domestication. Nevertheless, STRUCTURE analyses using microsatellite data and mtDNA sequences indicated the presence of two genetic pools in many populations, although a plausible explanation for this observation requires further studies.

PMID:
28407192
DOI:
10.4238/gmr16029608
[Indexed for MEDLINE]

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