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Toxins (Basel). 2017 Apr 13;9(4). pii: E137. doi: 10.3390/toxins9040137.

An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip.

Ouyang S1,2,3,4, Zhang Z5,6,7,8, He T9,10,11, Li P12,13,14,15, Zhang Q16,17, Chen X18,19, Wang D20,21, Li H22,23, Tang X24,25, Zhang W26,27,28.

Author information

1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. lyracsui@163.com.
2
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. lyracsui@163.com.
3
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. lyracsui@163.com.
4
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China. lyracsui@163.com.
5
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. zwzhang@whu.edu.cn.
6
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. zwzhang@whu.edu.cn.
7
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. zwzhang@whu.edu.cn.
8
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China. zwzhang@whu.edu.cn.
9
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. htnanobody@163.com.
10
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. htnanobody@163.com.
11
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. htnanobody@163.com.
12
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. peiwuli@oilcrops.cn.
13
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. peiwuli@oilcrops.cn.
14
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. peiwuli@oilcrops.cn.
15
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China. peiwuli@oilcrops.cn.
16
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. zhangqi521@126.com.
17
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. zhangqi521@126.com.
18
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. chenxiaomei_200870@126.com.
19
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China. chenxiaomei_200870@126.com.
20
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. wang416929@126.com.
21
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. wang416929@126.com.
22
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. lihui-gf@163.com.
23
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. lihui-gf@163.com.
24
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. wtxqtutu@163.com.
25
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China. wtxqtutu@163.com.
26
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. zhangwen@oilcrops.cn.
27
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. zhangwen@oilcrops.cn.
28
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China. zhangwen@oilcrops.cn.

Abstract

An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAbAFT) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAbAFT. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out durations were within 10 min. The recoveries for AFT in rice and peanut sample matrices were recorded from 86.25% to 118.0%, with relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety.

KEYWORDS:

agro-food safety; on-site; peanut and rice; quantum dot nanobead; test strip; total aflatoxin

PMID:
28406451
PMCID:
PMC5408211
DOI:
10.3390/toxins9040137
[Indexed for MEDLINE]
Free PMC Article

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