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J Am Soc Mass Spectrom. 2017 Aug;28(8):1733-1736. doi: 10.1007/s13361-017-1657-4. Epub 2017 Apr 12.

Simple and Environmentally Friendly Fabrication of Superhydrophobic Alkyl Ketene Dimer Coated MALDI Concentration Plates.

Author information

1
KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Analytical Chemistry, Stockholm, Sweden.
2
KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Organic Chemistry, Stockholm, Sweden.
3
Biotage Sweden AB, Uppsala, Sweden.
4
KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Analytical Chemistry, Stockholm, Sweden. aae@kth.se.

Abstract

Here we present a method to manufacture peptide-concentrating MALDI-plates with alkyl ketene dimer (AKD) as a new superhydrophobic coating. The fabrication of the hydrophobic plates included application of AKD by airbrush, and negative contact printing to generate the concentration sites. Deposited sample droplets were contained within the prestructured sites, and self-adjusted onto the site if slightly misplaced. No AKD contamination was observed, and the plates could easily be cleaned and regenerated. The S/N values for four model peptides was about twice as high compared with a standard steel plate and a commercial concentration plate. Graphical Abstract ᅟ.

KEYWORDS:

Alkyl ketene dimer; Concentration plates; MALDI

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