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Bio Protoc. 2017 Apr 5;7(7). pii: e2215. doi: 10.21769/BioProtoc.2215.

In vivo Mitophagy Monitoring in Caenorhabditis elegans to Determine Mitochondrial Homeostasis.

Author information

1
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
2
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece.

Abstract

Perturbation of mitochondrial function is a major hallmark of several pathological conditions and ageing, underlining the essential role of fine-tuned mitochondrial activity (Lopez-Otin et al., 2013). Mitochondrial selective autophagy, known as mitophagy, mediates the removal of dysfunctional and/or superfluous organelles, preserving cellular and organismal homeostasis (Palikaras and Tavernarakis, 2014; Pickrell and Youle, 2015; Scheibye-Knudsen et al., 2015). In this protocol, we describe a method for assessing mitophagy in the nematode Caenorhabditis elegans.

KEYWORDS:

Ageing; Autophagosome; Autophagy; Caenorhabditis elegans; Lysosomes; Mitochondria; Mitophagy; mtRosella

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