Format

Send to

Choose Destination
Genes Dev. 2017 Mar 15;31(6):617-627. doi: 10.1101/gad.292409.116.

Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote.

Author information

1
BIOSS Centre for Biological Signaling Studies, Faculty of Biology, Albert-Ludwigs-Universität Freiburg, 79104 Freiburg, Germany.
2
Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
3
Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
4
Laboratory of Plant Growth Regulation, Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0192, Japan.
5
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
6
Center for Plant Systems Biology, Vlaams Instituut voor Biotechnologie (VIB), 9052 Ghent, Belgium.
7
Medical Biotechnology Center, VIB, B-9000 Ghent, Belgium.
8
Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium.
9
Exploratory Research for Advanced Technology (ERATO), Japan Science and Tech Agency (JST), Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
10
CREST, JST, Ikoma, Nara 630-0192 Japan.

Abstract

In many plants, the asymmetric division of the zygote sets up the apical-basal axis of the embryo. Unlike animals, plant zygotes are transcriptionally active, implying that plants have evolved specific mechanisms to control transcriptional activation of patterning genes in the zygote. In Arabidopsis, two pathways have been found to regulate zygote asymmetry: YODA (YDA) mitogen-activated protein kinase (MAPK) signaling, which is potentiated by sperm-delivered mRNA of the SHORT SUSPENSOR (SSP) membrane protein, and up-regulation of the patterning gene WOX8 by the WRKY2 transcription factor. How SSP/YDA signaling is transduced into the nucleus and how these pathways are integrated have remained elusive. Here we show that paternal SSP/YDA signaling directly phosphorylates WRKY2, which in turn leads to the up-regulation of WOX8 transcription in the zygote. We further discovered the transcription factors HOMEODOMAIN GLABROUS11/12 (HDG11/12) as maternal regulators of zygote asymmetry that also directly regulate WOX8 transcription. Our results reveal a framework of how maternal and paternal factors are integrated in the zygote to regulate embryo patterning.

KEYWORDS:

MAPK signaling; WOX8; maternal factors; zygote asymmetry

PMID:
28404632
PMCID:
PMC5393056
DOI:
10.1101/gad.292409.116
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center