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Probiotics Antimicrob Proteins. 2018 Jun;10(2):251-257. doi: 10.1007/s12602-017-9276-8.

A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens α-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis.

Author information

1
Department of Molecular Microbiology, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
2
Department of Anaerobic Bacterial Vaccine Research and Production, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
3
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Post Box: 76169-133, Kerman, 7636161313, Iran.
4
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Post Box: 76169-133, Kerman, 7636161313, Iran. golchin@uk.ac.ir.
5
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

Abstract

The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin ( cpa 247-370 ) was synthesized and cloned into pT1NX vector to construct the pT1NX-alpha plasmid. This surface-expressing plasmid was electroporated into Lactobacillus casei ATCC 393, generating the recombinant L. casei strain expressing alpha-toxoid (LC-α strain). Expression of this modified alpha-toxoid was confirmed by SDS-PAGE, immunoblotting, and direct immunofluorescence microscopy. BALB/c mice, immunized orally by the recombinant LC-α strain, elicited mucosal and significantly humoral immune responses (p < 0.05) and developed a protection against 900 MLD/mL of the standard alpha-toxin. This study showed that this recombinant LC-α strain could be a promising vaccine candidate against gas gangrene and necrotic enteritis.

KEYWORDS:

Clostridium perfringens; Lactobacillus casei; Probiotic; Toxoid; Vaccine

PMID:
28401477
DOI:
10.1007/s12602-017-9276-8
[Indexed for MEDLINE]

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