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Mol Cell Probes. 2017 Aug;34:1-12. doi: 10.1016/j.mcp.2017.04.002. Epub 2017 Apr 8.

Umbilical cord mesenchymal stem cells are able to undergo differentiation into functional islet-like cells in type 2 diabetic tree shrews.

Author information

1
Cell Biological Therapy Center of Kunming General Hospital of Chengdu Military Command, Kunming, Yunnan Province, China; The Nation and Region Integrated Engineering Laboratory of Stem Cell and Immunocyte Biological Technology, Kunming, Yunnan Province, China; Key Laboratory of Translational Medicine of Cell Therapy Technology of Yunnan Province, Kunming, Yunnan, China.
2
Cell Biological Therapy Center of Kunming General Hospital of Chengdu Military Command, Kunming, Yunnan Province, China.
3
Key Laboratory of Translational Medicine of Cell Therapy Technology of Yunnan Province, Kunming, Yunnan, China.
4
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650093, Yunnan, China.
5
The Nation and Region Integrated Engineering Laboratory of Stem Cell and Immunocyte Biological Technology, Kunming, Yunnan Province, China.
6
Department of Occupational Medicine, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, Guangdong, China.
7
Key Laboratory of Translational Medicine of Cell Therapy Technology of Yunnan Province, Kunming, Yunnan, China. Electronic address: chenyidoctor@sohu.com.
8
Cell Biological Therapy Center of Kunming General Hospital of Chengdu Military Command, Kunming, Yunnan Province, China; The Nation and Region Integrated Engineering Laboratory of Stem Cell and Immunocyte Biological Technology, Kunming, Yunnan Province, China; Key Laboratory of Translational Medicine of Cell Therapy Technology of Yunnan Province, Kunming, Yunnan, China. Electronic address: qing1021zhu@163.com.

Abstract

Islet transplantation is arguably one of the most promising strategies to treat patients suffering with diabetes mellitus. However, a combination of a lack of donors and chronic immune rejection limit clinical applications. Here, we evaluated the efficacy of cell therapy using islet-like cells differentiated from umbilical cord mesenchymal stem cells (UC-MSCs) of tree shrews for the treatment of type 2 diabetes. Enhanced green fluorescent protein (eGFP) labeled UC-MSCs were directly injected into type 2 diabetic tree shrews, where UC-MSC differentiated into functional islet-like cells and alleviated disease severity, as evidenced by improved biochemical features and reduced concentrations of inflammatory cytokines. We also demonstrated that in vitro culture of UC-MSCs for six days in a high-glucose environment (40 mmol/L or 60 mmol/L glucose) resulted in significant gene methylation. The potency of UC-MSCs differentiated into insulin-secreting cells was attributed to the activation of Notch signal pathways. This study provides evidence that cell therapy of islet-like cells differentiated from UC-MSCs is a feasible, simple and inexpensive approach in the treatment of type 2 diabetes.

KEYWORDS:

Diabetes; Insulin-secreting cells; Mesenchymal stem cells; Umbilical cord

PMID:
28400333
DOI:
10.1016/j.mcp.2017.04.002
[Indexed for MEDLINE]

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