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Curr Protoc Neurosci. 2017 Apr 10;79:8.39.1-8.39.20. doi: 10.1002/cpns.23.

Systems Genetic Analysis in GeneNetwork.org.

Author information

1
Department of Psychology and Program in Neuroscience, Middlebury College, Middlebury, Vermont.
2
Center for Mammalian Genetics, The Jackson Laboratory, Bar Harbor, Maine.
3
Center for Computational Sciences, The Jackson Laboratory, Bar Harbor, Maine.

Abstract

Genome-wide association studies (GWAS) have emerged as a powerful tool to identify alleles and molecular pathways that influence susceptibility to psychiatric disorders and other diseases. Forward genetics using mouse mapping populations allows for a complementary approach that provides rigorous genetic and environmental control. In this unit, we describe techniques and tools that reduce the technical burden traditionally associated with genetic mapping in mice and enhance their translational utility to human psychiatric disorders. We provide guidance on choosing the appropriate mapping population, discuss the importance of phenotype, and offer detailed instructions on using the Web-based resource GeneNetwork to aid neuroscientists in better understanding the mechanisms through which genes influence behavior. We believe that the continued development of mouse mapping populations, genetic tools, bioinformatics resources, and statistical methodologies should remain a parallel strategy by which to investigate the genetic and environmental underpinnings of psychiatric disorders and other diseases in humans.

KEYWORDS:

GeneNetwork; quantitative trait locus mapping; recombinant inbred strains; systems genetics

PMID:
28398643
PMCID:
PMC5548442
DOI:
10.1002/cpns.23
[Indexed for MEDLINE]
Free PMC Article

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