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Nat Commun. 2017 Apr 11;8:15028. doi: 10.1038/ncomms15028.

Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models.

Author information

1
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore.
2
NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore 117456, Singapore.
3
Department of Biological and Environmental Engineering, Cornell University, Ithaca, New York 14853, USA.
4
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
5
Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore S 119074, Singapore.

Abstract

Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro. Here, we generate a modified version of the system, including a gene encoding an anti-biofilm enzyme, and use the probiotic strain Escherichia coli Nissle 1917 as host. The engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice). These findings support the further development of engineered microorganisms with potential prophylactic and therapeutic activities against gut infections.

PMID:
28398304
PMCID:
PMC5394271
DOI:
10.1038/ncomms15028
[Indexed for MEDLINE]
Free PMC Article

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