Send to

Choose Destination
Sci Rep. 2017 Apr 10;7(1):766. doi: 10.1038/s41598-017-00774-9.

Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells.

Author information

Wilmer Eye Institute, The Johns Hopkins Wilmer Eye Institute 600 N. Wolfe Street, Baltimore, MD, 21287, USA.
Shiley Eye Institute, University of California San Diego, La Jolla, California, USA.
Is PhenoCell, Evry, France.
Retinal Neurophysiology Section, National Eye Institute, Bethesda, MD, USA.
Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.
Wilmer Eye Institute, The Johns Hopkins Wilmer Eye Institute 600 N. Wolfe Street, Baltimore, MD, 21287, USA.
Department of Molecular Biology and Genetics, Neuroscience, and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.


The retinal degenerative diseases, which together constitute a leading cause of hereditary blindness worldwide, are largely untreatable. Development of reliable methods to culture complex retinal tissues from human pluripotent stem cells (hPSCs) could offer a means to study human retinal development, provide a platform to investigate the mechanisms of retinal degeneration and screen for neuroprotective compounds, and provide the basis for cell-based therapeutic strategies. In this study, we describe an in vitro method by which hPSCs can be differentiated into 3D retinas with at least some important features reminiscent of a mature retina, including exuberant outgrowth of outer segment-like structures and synaptic ribbons, photoreceptor neurotransmitter expression, and membrane conductances and synaptic vesicle release properties consistent with possible photoreceptor synaptic function. The advanced outer segment-like structures reported here support the notion that 3D retina cups could serve as a model for studying mature photoreceptor development and allow for more robust modeling of retinal degenerative disease in vitro.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center