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Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3385-E3389. doi: 10.1073/pnas.1700141114. Epub 2017 Apr 10.

Automated glycan assembly using the Glyconeer 2.1 synthesizer.

Author information

1
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
2
Institute of Chemistry and Biochemistry, Free University Berlin, 14195 Berlin, Germany.
3
GlycoUniverse GmbH & Co. KGaA, 14195 Berlin, Germany.
4
Fritz Haber Institute of the Max Planck Society, 14195 Berlin, Germany.
5
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany; peter.seeberger@mpikg.mpg.de.

Abstract

Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.

KEYWORDS:

Glyconeer; automated synthesis; glycobiology; glycochemistry; oligosaccharide synthesis

Conflict of interest statement

Conflict of interest statement: P.H.S. declares a significant financial interest in GlycoUniverse GmbH & Co. KGaA, the company that commercializes the synthesis instrument, building blocks, and other reagents.

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