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Stem Cell Res. 2017 Jan;18:22-25. doi: 10.1016/j.scr.2016.12.006. Epub 2016 Dec 9.

Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions.

Author information

1
Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
2
Center of Neurodevelopmental Disorders at Karolinska Institutet, Pediatric Neuropsychiatry Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden; Center of Psychiatry Research, Stockholm County Council, Stockholm, Sweden.
3
Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden. Electronic address: anna.falk@ki.se.

Abstract

Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.

PMID:
28395796
DOI:
10.1016/j.scr.2016.12.006
[Indexed for MEDLINE]
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