Format

Send to

Choose Destination
Stem Cell Res. 2017 Apr;20:50-53. doi: 10.1016/j.scr.2017.02.007. Epub 2017 Feb 24.

Generation and characterization of two iPSC lines from human epicardium-derived cells.

Author information

1
Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
2
Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
3
Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany. Electronic address: schrader@uni-duesseldorf.de.

Abstract

Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.

PMID:
28395740
DOI:
10.1016/j.scr.2017.02.007
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center