Format

Send to

Choose Destination
Nat Chem Biol. 2017 Jun;13(6):655-659. doi: 10.1038/nchembio.2348. Epub 2017 Apr 10.

Mechanistic insights into energy conservation by flavin-based electron bifurcation.

Author information

1
Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado, USA.
2
School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
3
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
4
Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA.
5
Department of Chemistry, University of Kentucky, Lexington, Kentucky, USA.
6
Institute of Biological Chemistry, Washington State University, Pullman, Washington, USA.

Abstract

The recently realized biochemical phenomenon of energy conservation through electron bifurcation provides biology with an elegant means to maximize utilization of metabolic energy. The mechanism of coordinated coupling of exergonic and endergonic oxidation-reduction reactions by a single enzyme complex has been elucidated through optical and paramagnetic spectroscopic studies revealing unprecedented features. Pairs of electrons are bifurcated over more than 1 volt of electrochemical potential by generating a low-potential, highly energetic, unstable flavin semiquinone and directing electron flow to an iron-sulfur cluster with a highly negative potential to overcome the barrier of the endergonic half reaction. The unprecedented range of thermodynamic driving force that is generated by flavin-based electron bifurcation accounts for unique chemical reactions that are catalyzed by these enzymes.

PMID:
28394885
DOI:
10.1038/nchembio.2348
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center