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ACS Omega. 2017 Mar 31;2(3):994-1002. doi: 10.1021/acsomega.6b00395. Epub 2017 Mar 16.

Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes.

Author information

1
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford , Oxford OX3 7DQ, U.K.
2
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, U.K.; Mechatronics and Bioengineering Science Research Groups, Faculty of Engineering and the Environment, University of Southampton, SO17 1BJ Southampton, U.K.
3
Weatherall Institute of Molecular Medicine, MRC Human Immunology Unit, University of Oxford , Oxford OX3 9DS, U.K.
4
Rutherford Appleton Laboratory, Central Laser Facility, Science and Technology Facilities Council, Research Complex at Harwell , Harwell-Oxford, Didcot OX11 0FA, U.K.

Abstract

Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles. Currently, however, it is expensive and time-consuming compared with other methods. In this study, we demonstrate that 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes, such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.

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