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Int J Biochem Cell Biol. 2017 Jun;87:86-94. doi: 10.1016/j.biocel.2017.04.002. Epub 2017 Apr 6.

Neurochemical effects of the R form of α-lipoic acid and its neuroprotective mechanism in cellular models of Parkinson's disease.

Author information

1
Department of Neurology, The Fourth Affiliate Clinical Hospital, Harbin Medical University, Harbin 150001 China. Electronic address: hongzhao113@hotmail.com.
2
Department of Neurology, The Fourth Affiliate Clinical Hospital, Harbin Medical University, Harbin 150001 China.

Abstract

OBJECTIVE:

Parkinson's disease (PD) is a common neurodegenerative disease. This study aimed to investigate the effects of the R form of α-lipoic acid (RLA) in cellular models of PD induced by 6-hydroxydopamine (6-OHDA) or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).

METHODS:

Cell viability and apoptosis were detected using CCK8 and Annexin V-FITC assays, respectively. Intracellular reactive oxygen species (ROS) were detected by fluorescence staining. ELISA assays were performed to detect the levels of dopamine and α-synuclein. To evaluate the effects of RLA on mitochondrial function, cytotoxicity, ATP levels, and mitochondrial gene expression were assayed. Additionally, the expression levels of autophagy-related proteins, including Parkin, PINK1, p62, ATG12, and LC3, were analyzed by western blot, and cell autophagy was visualized by immunofluorescence.

RESULTS:

RLA increased cell viability and decreased apoptosis, intracellular ROS, and cytotoxicity, and induced cell autophagy in PD models induced by 6-OHDA and MPTP. RLA also reversed the decreased dopamine and increased α-synuclein expression induced by 6-OHDA and MPTP. The mitochondrial regulatory protein PGC-1α was significantly up-regulated by RLA. The expression levels of autophagy-related proteins, including Parkin, PINK1, p62, and ATG12, were down-regulated after RLA treatment, while LC3 expression was up-regulated.

CONCLUSIONS:

RLA has a protective effect against cellular damage induced by 6-OHDA and MPTP. The neuroprotective mechanism of RLA may be associated with improvement of mitochondrial function and autophagy. Therefore, RLA may serve as a promising potential adjuvant for PD treatment.

KEYWORDS:

Neuroprotection; Neurotoxin; Parkinson’s disease; R form α-lipoic acid; SH-SY5Y cells

PMID:
28390981
DOI:
10.1016/j.biocel.2017.04.002
[Indexed for MEDLINE]

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