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J Nanobiotechnology. 2017 Apr 7;15(1):27. doi: 10.1186/s12951-017-0263-8.

Biodistribution of arctigenin-loaded nanoparticles designed for multimodal imaging.

Author information

1
College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, People's Republic of China.
2
State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin, 300071, China.
3
Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Department of Physiology, School of Medicine, Nankai University, Tianjin, 300071, China.
4
State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin, 300071, China. xzfeng@nankai.edu.cn.
5
College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, People's Republic of China. gangbai@nankai.edu.cn.

Abstract

BACKGROUND:

Tracking targets of natural products is one of the most challenging issues in fields ranging from pharmacognosy to biomedicine. It is widely recognized that the biocompatible nanoparticle (NP) could function as a "key" that opens the target "lock".

RESULTS:

We report a functionalized poly-lysine NP technique that can monitor the target protein of arctigenin (ATG) in vivo non-invasively. The NPs were synthesized, and their morphologies and surface chemical properties were characterized by transmission electron microscopy (TEM), laser particle size analysis and atomic force microscopy (AFM). In addition, we studied the localization of ATG at the level of the cell and the whole animal (zebrafish and mice). We demonstrated that fluorescent NPs could be ideal carriers in the development of a feasible method for target identification. The distributions of the target proteins were found to be consistent with the pharmacological action of ATG at the cellular and whole-organism levels.

CONCLUSIONS:

The results indicated that functionalized poly-lysine NPs could be valuable in the multimodal imaging of arctigenin.

KEYWORDS:

Arctigenin; Localization; Nanoparticles; Self-assembly; Target identification

PMID:
28388905
PMCID:
PMC5383946
DOI:
10.1186/s12951-017-0263-8
[Indexed for MEDLINE]
Free PMC Article

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