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Pediatr Res. 2017 Sep;82(3):509-517. doi: 10.1038/pr.2017.99. Epub 2017 May 17.

Influence of antenatal glucocorticoid on preterm lamb diaphragm.

Author information

1
School of Human Sciences, The University of Western Australia, Perth, Western Australia, Australia.
2
Centre for Neonatal Research and Education, Division of Paediatrics and Child Health, Medical School, The University of Western Australia, Perth, Western Australia, Australia.
3
School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia.
4
School of Public Health, Curtin University, Perth, Western Australia, Australia.
5
Centre for Genetic Origins of Health and Disease, The University of Western Australia and Curtin University, Perth, Western Australia, Australia.

Abstract

BackgroundPregnant women at a high risk of preterm delivery receive glucocorticoids to accelerate fetal lung maturation and surfactant synthesis. However, the effect of antenatal steroids on the developing diaphragm remains unclear. We hypothesized that maternal betamethasone impairs the fetal diaphragm, and the magnitude of the detrimental effect increases with longer duration of exposure. We aimed to determine how different durations of fetal exposure to maternal betamethasone treatment influence the fetal diaphragm at the functional and molecular levels.MethodsDate-mated merino ewes received intramuscular injections of saline (control) or two doses of betamethasone (5.7 mg) at an interval of 24 h commencing either 2 or 14 days before delivery. Preterm lambs were killed after cesarean delivery at 121-day gestational age. In vitro contractile measurements were performed on the right hemidiaphragm, whereas molecular/cellular analyses used the left costal diaphragm.ResultsDifferent durations of fetal exposure to maternal betamethasone had no consistent effect on the protein metabolic pathway, expression of glucocorticoid receptor and its target genes, cellular oxidative status, or contractile properties of the fetal lamb diaphragm.ConclusionThese data suggest that the potential benefits of betamethasone exposure on preterm respiratory function are not compromised by impaired diaphragm function after low-dose maternal intramuscular glucocorticoid exposure.

PMID:
28388600
DOI:
10.1038/pr.2017.99
[Indexed for MEDLINE]

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