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J Cell Physiol. 2018 Feb;233(2):990-1004. doi: 10.1002/jcp.25947. Epub 2017 May 24.

Chick derived induced pluripotent stem cells by the poly-cistronic transposon with enhanced transcriptional activity.

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Ecological Risk Assessment and Control Section, Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, Japan.
Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Japan.
Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan.
Department of Obstetrics and Gynecology, Juntendo University, Hongo, Bunkyo-ku, Tokyo, Japan.
Laboratory of Animal Reproduction, Department of Agriculture, Kindai University, Nara, Japan.
United Graduate School of Agricultural Sciences, Iwate University, Ueda, Morioka, Japan.


Induced pluripotent stem (iPS) cell technology lead terminally differentiated cells into the pluripotent stem cells through the expression of defined reprogramming factors. Although, iPS cells have been established in a number of mammalian species, including mouse, human, and monkey, studies on iPS cells in avian species are still very limited. To establish chick iPS cells, six factors were used within the poly-cistronic reprogramming vector (PB-R6F), containing M3O (MyoD derived transactivation domain fused with Oct3/4), Sox2, Klf4, c-Myc, Lin28, and Nanog. The PB-R6F derived iPS cells were alkaline-phosphatase and SSEA-1 positive, which are markers of pluripotency. Elevated levels of endogenous Oct3/4 and Nanog genes were detected in the established iPS cells, suggesting the activation of the FGF signaling pathway is critical for the pluripotent status. Histological analysis of teratoma revealed that the established chick iPS cells have differentiation ability into three-germ-layer derived tissues. This is the first report of establishment of avian derived iPS cells with a single poly-cistronic transposon based expression system. The establishment of avian derived iPS cells could contribute to the genetic conservation and modification of avian species.


avian; induced pluripotent stem (iPS) cells; pluripotent network; primed type stem cell

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