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Monoclon Antib Immunodiagn Immunother. 2017 Apr;36(2):77-79. doi: 10.1089/mab.2017.0002. Epub 2017 Apr 7.

Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin.

Author information

1
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
2
2 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
3
3 Graduate School of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
4
4 New Industry Creation Hatchery Center, Tohoku University , Sendai, Japan .

Abstract

Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb. Immunohistochemical analysis shows that mPMab-1 detects podocytes of the kidney, lymphatic endothelial cells of the colon, and type I alveolar cells of the lung. Importantly, mPMab-1 is more sensitive than PMab-1. This conversion strategy from rat mAb to mouse mAb could be applicable to other mAbs.

KEYWORDS:

PMab-1; chimeric antibody; monoclonal antibody; podoplanin

PMID:
28387612
DOI:
10.1089/mab.2017.0002
[Indexed for MEDLINE]

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