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J Interferon Cytokine Res. 2017 Apr;37(4):175-180. doi: 10.1089/jir.2016.0106.

Interleukin-10 and Interferon Gamma Gene Polymorphisms and Hepatitis C Virus-Related Liver Cirrhosis Risk.

Author information

1
1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University , Sohag, Egypt .
2
2 Department of Tropical Medicine and Gastroenterology, Faculty of Medicine, Sohag University , Sohag, Egypt .
3
3 Department of Medical Biochemistry, Faculty of Medicine, Sohag University , Sohag, Egypt .
4
4 Department of Physiology, Faculty of Medicine, Sohag University , Sohag, Egypt .
5
5 Department of Internal Medicine, Faculty of Medicine, Sohag University , Sohag, Egypt .

Abstract

The aim of the study was to evaluate the association between the gene polymorphisms in interleukin-10 (IL-10) and interferon gamma (IFN-γ) genes with susceptibility and severity of hepatitis C virus (HCV) infection among Egyptian patients. Interleukin-10 -592 A/C, -1082 G/A and IFN-γ +874 T/A genotypes were determined in 100 chronic HCV patients and 50 healthy controls using restriction fragment length polymorphism (RFLP) and the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) respectively. IL-10 -592 A/C polymorphism genotyping revealed that the frequency of CC genotype was significantly higher in chronic HCV patients than in controls (58% versus 30%, P < 0.05). Regarding IL-10 -1082 G/A polymorphism genotyping, a higher frequency of GG genotype was found in chronic HCV patients compared to controls (31% versus 10%, P < 0.05). IFN-γ +874 T/A genotyping showed that TT genotype was significantly higher in chronic HCV participants than controls (31% versus 18%, P < 0.05), while a higher frequency of T allele was found in cirrhotic patients compared to noncirrhotic patients (P < 0.05). Our observations suggested that IL-10 -592 A/C, -1082 G/A, and IFN-γ +874 T/A polymorphisms had a strong association with susceptibility to HCV infection. However, no significant association was observed between the cytokines (IL-10 and IFN-γ) genotypes profile and HCV-liver cirrhosis risk in the studied population, except for the high frequency of IFN-γ +874 T allele in cirrhotic patients.

KEYWORDS:

gene polymorphism; hepatitis C virus; interferon-γ; interleukin-10

PMID:
28387594
DOI:
10.1089/jir.2016.0106
[Indexed for MEDLINE]

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