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Am J Transl Res. 2017 Mar 15;9(3):1213-1221. eCollection 2017.

miR-613 inhibits bladder cancer proliferation and migration through targeting SphK1.

Author information

1
Department of Urology, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, Zhejiang, China.
2
Department of Obstetric and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, Zhejiang, China.

Abstract

OBJECTIVES:

Increasing evidence has suggested that microRNA (miRNA) dysregulation may contribute to tumor progression and metastasis. However, the role of miR-613 in bladder cancer was still unknown.

MATERIALS AND METHODS:

qRT-PCR and Western blotting were performed to detect the expression of miR-613 and its direct target gene. CCK-8 analysis, qRT-PCR and cell invasion were performed to measure the cell function.

RESULTS:

We demonstrated that the expression of miR-613 was downregulated in the bladder cancer cell lines. In addition, miR-613 expression was downregulated in the bladder cancer tissues compared to the adjacent normal tissues. Out of 35 bladder cancer tissues, miR-613 was downregulated in 27 cases compared to the adjacent tissues. Ectopic expression of miR-613 suppressed the bladder cancer cell proliferation and invasion. Moreover, miR-613 overexpression enhanced the expression of epithelial biomarker, Ecadherin, and suppressed the expression of mesenchymal biomarker, Vimentin, Snail and N-cadherin. Furthermore, we identified the Sphingosine kinase 1 (SphK1) as the direct target gene of miR-613 in the bladder cancer cell. Restoration of Sphk1 partially rescued miR-613-inhibited bladder cancer cell proliferation, invasion and EMT.

CONCLUSIONS:

These data suggested that miR-613 acted a tumor suppressive role in bladder cancer through targeting SphK1 in bladder.

KEYWORDS:

Bladder cancer; SphK1; miR-613; miRNAs; microRNAs

PMID:
28386347
PMCID:
PMC5376012

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