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Front Neural Circuits. 2017 Mar 23;11:19. doi: 10.3389/fncir.2017.00019. eCollection 2017.

NCAM Regulates Inhibition and Excitability in Layer 2/3 Pyramidal Cells of Anterior Cingulate Cortex.

Author information

1
Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill Chapel Hill, NC, USA.
2
Department of Otolaryngology/Head and Neck Surgery, The University of North Carolina at Chapel Hill Chapel Hill, NC, USA.
3
Department of Otolaryngology/Head and Neck Surgery, The University of North Carolina at Chapel HillChapel Hill, NC, USA; Department of Cell Biology and Physiology, The University of North Carolina at Chapel HillChapel Hill, NC, USA.

Abstract

The neural cell adhesion molecule (NCAM), has been shown to be an obligate regulator of synaptic stability and pruning during critical periods of cortical maturation. However, the functional consequences of NCAM deletion on the organization of inhibitory circuits in cortex are not known. In vesicular gamma-amino butyric acid (GABA) transporter (VGAT)-channelrhodopsin2 (ChR2)-enhanced yellow fluorescent protein (EYFP) transgenic mice, NCAM is expressed postnatally at perisomatic synaptic puncta of EYFP-labeled parvalbumin, somatostatin and calretinin-positive interneurons, and in the neuropil in the anterior cingulate cortex (ACC). To investigate how NCAM deletion affects the spatial organization of inhibitory inputs to pyramidal cells, we used laser scanning photostimulation in brain slices of VGAT-ChR2-EYFP transgenic mice crossed to either NCAM-null or wild type (WT) mice. Laser scanning photostimulation revealed that NCAM deletion increased the strength of close-in inhibitory connections to layer 2/3 pyramidal cells of the ACC. In addition, in NCAM-null mice, the intrinsic excitability of pyramidal cells increased, whereas the intrinsic excitability of GABAergic interneurons did not change. The increase in inhibitory tone onto pyramidal cells, and the increased pyramidal cell excitability in NCAM-null mice will alter the delicate coordination of excitation and inhibition (E/I coordination) in the ACC, and may be a factor contributing to circuit dysfunction in diseases such as schizophrenia and bipolar disorder, in which NCAM has been implicated.

KEYWORDS:

brain slice; channelrhodopsin; laser scanning photostimulation; mouse; patch clamp

PMID:
28386219
PMCID:
PMC5362729
DOI:
10.3389/fncir.2017.00019
[Indexed for MEDLINE]
Free PMC Article

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